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primary anti k14 antibody  (Proteintech)


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    Proteintech primary anti k14 antibody
    Primary Anti K14 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+anti+k14+antibody/Cytokeratin+14+Antibody/pmc12281451-186-6-18
    Average 97 stars, based on 187 article reviews
    primary anti k14 antibody - by Bioz Stars, 2026-09
    97/100 stars

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    other:

    Article Title: Biomimetic gender-specific human skin model based on gonads/epidermis-on-a-chip
    Article Snippet: Primary anti-Ki67 antibody, primary anti-K10 antibody, primary anti-K14 antibody, Actin-Tracker Green-488, and fluorophore-conjugated secondary antibodies were brought from Proteintech.



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    (A) Immunohistochemical analysis using antibodies against six epidermal differentiation marker proteins (cytokeratin 1, 14, 6, involucrin, <t>filaggrin</t> and, loricrin). The sections were harvest from neonate epidermis of control, HK1.bcl-2, HK1.v-Ha-ras, and HK1.bcl-2/HK1.v-Ha-ras transgenic mice. (B) Western blot analysis of epidermal protein extracted from neonatal skins.
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    Image Search Results


    Optimal conditions for HFLS formation. ( A ) The time interval between the SV40T-hTERT DPc seeding for the spheroid formation and the start of co-culture with ORSc; approximately 1000 cells of SV40T-hTERT DPc and 3000 cells of ORSc were used. ( B ) Regulating co-cultured ORSc: Elongation of the keratinocyte layer in HFLS is prominent in the range of 3000–4000 ORSc. ( C ) Regulating immortalized keratinocyte (Ker-CT) cell numbers in HFLS composed of immortalized cells only: Addition of more keratinocytes results in larger iHFLS. ( D ) Variation of iHFLS formation according to culture medium regulation: iHFLS cultured in William’s E medium exhibits a more linear form than in other media conditions. ( E ) The cell arrangement in iHFLS using fluorescence observation: Cellular arrangement similar to hair follicles was observed using a cell tracker attached on SV40T-hTERT DPc and immunostaining of keratin 14 (K14) keratinocyte biomarker.

    Journal: Bioengineering

    Article Title: Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds

    doi: 10.3390/bioengineering9110645

    Figure Lengend Snippet: Optimal conditions for HFLS formation. ( A ) The time interval between the SV40T-hTERT DPc seeding for the spheroid formation and the start of co-culture with ORSc; approximately 1000 cells of SV40T-hTERT DPc and 3000 cells of ORSc were used. ( B ) Regulating co-cultured ORSc: Elongation of the keratinocyte layer in HFLS is prominent in the range of 3000–4000 ORSc. ( C ) Regulating immortalized keratinocyte (Ker-CT) cell numbers in HFLS composed of immortalized cells only: Addition of more keratinocytes results in larger iHFLS. ( D ) Variation of iHFLS formation according to culture medium regulation: iHFLS cultured in William’s E medium exhibits a more linear form than in other media conditions. ( E ) The cell arrangement in iHFLS using fluorescence observation: Cellular arrangement similar to hair follicles was observed using a cell tracker attached on SV40T-hTERT DPc and immunostaining of keratin 14 (K14) keratinocyte biomarker.

    Article Snippet: The slides were blocked with bovine serum albumin (GenDEPOT, Katy, TX, USA) and incubated with anti-K14 primary antibody (Santa Cruz Biotechnology, Dallas, TX, USA) at 4 °C overnight, and then with secondary antibody (Molecular Probes, Eugene, OR, USA).

    Techniques: Co-Culture Assay, Cell Culture, Fluorescence, Immunostaining, Biomarker Discovery

    (A) Immunohistochemical analysis using antibodies against six epidermal differentiation marker proteins (cytokeratin 1, 14, 6, involucrin, filaggrin and, loricrin). The sections were harvest from neonate epidermis of control, HK1.bcl-2, HK1.v-Ha-ras, and HK1.bcl-2/HK1.v-Ha-ras transgenic mice. (B) Western blot analysis of epidermal protein extracted from neonatal skins.

    Journal: The American Journal of dermatopathology

    Article Title: Restrained Terminal Differentiation and Sustained Stemness in neonatal skin by Ha-Ras and Bcl-2

    doi: 10.1097/DAD.0000000000000678

    Figure Lengend Snippet: (A) Immunohistochemical analysis using antibodies against six epidermal differentiation marker proteins (cytokeratin 1, 14, 6, involucrin, filaggrin and, loricrin). The sections were harvest from neonate epidermis of control, HK1.bcl-2, HK1.v-Ha-ras, and HK1.bcl-2/HK1.v-Ha-ras transgenic mice. (B) Western blot analysis of epidermal protein extracted from neonatal skins.

    Article Snippet: For the skin differentiation study, rabbit anti-mouse primary antibodies, including K1, K6, K14, involucrin, loricrin and filaggrin (Babco, Berkeley, CA) were used.

    Techniques: Immunohistochemical staining, Marker, Control, Transgenic Assay, Western Blot